Isolation and Characterization of Terpenoids with Promising Biopesticide Activity from Dittrichia viscosa (L.) Roots Segura Navarro, María José Quílez Del Moral, José Francisco Galisteo, Alberto López Pérez, José Luis Molina Inzunza, Diego O. Andrés, María Fe de González Coloma, Azucena Fernández Barrero, Alejandro Dittrichia viscosa roots Terpenes Sesquiterpene lactones The natural product composition of the hexane and methyl tert-butyl ether extracts of Dittrichia viscosa roots was examined. Eight terpenoids were identified by nuclear magnetic resonance (NMR) and high resolution mass spectroscometry (HRMS) techniques, four of which (1, 5, 6 and 8) are reported here for the first time as natural products. Of these eight compounds, four are thymol derivatives (1–4), two are guaianolides (5 and 7) and two are himachalanes (6 and 8). Additionally, the occurrence of himachalanes in this species is reported for the first time. Furthermore, a study of the potential plant protection effects of some of these natural products and the chemical derivative 6a was carried out. Promising preliminary results were obtained for compounds 1–3 and 6a as antifeedant agents against Spodoptera littoralis; 1–3 and 5 against Myzus persicae; 1–3 against Rhopalosiphum padi; and 4 as nematicide against Meloidogyne javanica. Finally, the phytotoxic activity of compounds 4, 5 and 6a against the monocotyledonous species Lolium perenne was also proven. 2026-03-25T07:56:49Z 2026-03-25T07:56:49Z 2026-03-24 journal article Segura-Navarro, M. J., Quílez del Moral, J. F., Galisteo, A., López-Pérez, J. L., Molina Inzunza, D. O., Andrés, M. F., González-Coloma, A., & Barrero, A. F. (2026). Isolation and Characterization of Terpenoids with Promising Biopesticide Activity from Dittrichia viscosa (L.) Roots. International Journal of Molecular Sciences, 27(7), 2949. https://doi.org/10.3390/ijms27072949 https://hdl.handle.net/10481/112459 10.3390/ijms27072949 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI