Influence of the Topography of Zirconium Treated with Laser Micropatterning on Periodontal Ligament Stem Cells: An In Vitro Study Serrano-Belmonte, Ildefonso Rico Molina, Alba Rosales Leal, Juan Ignacio Lorite Méndez, Guillermo Rodríguez Valverde, Miguel Ángel Serna-Muñoz, Clara Martínez-Cánovas, Ascensión Zirconium Implant abutment Laser micropatterning Zirconium is a widely used material in the field of dentistry, employed for implants and their components as well as for the creation of crowns and veneers. Given that its biocompatibility has been studied and demonstrated in various fields of application, it is necessary to analyze how surface modification of this material influences its properties. The purpose of this study was to analyze the biocompatibility, initial adhesion (48 h), and morphology of periodontal ligament stem cells (PDLSCs) seeded on different zirconium surfaces treated with laser micropatterning, as well as plastic coverslips as a control. The Neubauer chamber was used to count the cells adhered to each of the sets, and confocal and scanning electron microscopy were employed to examine the adhesion and morphology of periodontal ligament stem cells on each of the zirconium surfaces studied. Results: Statistically significant differences were found in terms of primary cell adhesion, with sets 3 (grid topography) and 4 (channel topography) showing the most favorable characteristics for fibroblast adhesion. It was concluded that regular and moderately rough surfaces promoted better cell proliferation and development. 2026-03-10T10:55:18Z 2026-03-10T10:55:18Z 2026-03-09 journal article Serrano-Belmonte, I., Rico-Molina, A., Rosales-Leal, J. I., Lorite-Méndez, G., Rodríguez-Valverde, M. Á., Serna-Muñoz, C., & Martínez-Cánovas, A. (2026). Influence of the Topography of Zirconium Treated with Laser Micropatterning on Periodontal Ligament Stem Cells: An In Vitro Study. Journal of Functional Biomaterials, 17(3), 132. https://doi.org/10.3390/jfb17030132 https://hdl.handle.net/10481/112010 10.3390/jfb17030132 eng http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional MDPI