The effect of the microalgae-bacteria microbiome on wastewater treatment and biomass production Paddock, Matthew Fernández Bayo, Jesús Dionisio VanderGheynst, Jean S. algae wastewater microbial community The use of microalgae for wastewater treatment has been proposed as a cost-effective method to produce biofuels while remediating waste streams. This study examined the microalgae biomass production rate, wastewater treatment efficiency, and prokaryotic organism microbiome associated with microalgae Chlorella sorokiniana cultivated on anaerobic digestate effluent. Final microalgae biomass concentrations from nine photobioreactors were highly variable and had values that ranged between 0.14 g/L and 0.90 g/L. Nutrient removal efficiencies for TN (total nitrogen), N-NH4 (ammonium nitrogen), and COD (chemical oxygen demand) ranged from 34% to 67%, 65% to 97%, and−60% to 14%, respectively. Analysis of individual OTUs (operational taxonomic units) from the microbial community revealed that microalgae biomass concentrations were significantly correlated with the relative abundance of OTUs in the genus Pusillimonas. Predictive metagenomic analyses identified additional correlations associated with biomass production and nutrient removal. These results suggest that the microbial community present during microalgae cultivation on wastewater can impact the performance of the system for biomass production and wastewater treatment. 2026-02-23T08:20:55Z 2026-02-23T08:20:55Z 2020-01-01 journal article Matthew B. Paddock, Jesús Dionisio Fernández-Bayo, Jean S. VanderGheynst. (2020) The effect of the microalgae- bacteria microbiome on wastewater treatment and biomass production. Applied Microbiology and Biotechnology. 104, 893–905. https://doi.org/10.1007/s00253-019-10246-x https://hdl.handle.net/10481/111357 https://doi.org/10.1007/s00253-019-10246-x eng http://creativecommons.org/licenses/by-nc-nd/3.0/ embargoed access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License