Start-up of novel pilot-scale plant based on two-stage expanded granular sludge bed reactors for pig slurry treatment: Performance and microbial dynamics Aragón Barroso, Antonio J. Gallardo Altamirano, Manuel Jesús Castellano-Hinojosa, Antonio González Martínez, Alejandro González López, Jesús Juan Osorio Robles, Francisco Anaerobic digestion High-rate Biogas Pig manure management is an environmental challenge that can be improved through high-rate anaerobic digestion, enabling efficient biogas production and resource recovery. This study evaluated a pilot-scale two-stage expanded granular sludge bed (EGSB) system treating real pig slurry over a 150-day start-up. The reactors, operated in series with different working volumes (100 and 500 L), underwent progressive hydraulic retention times (HRT) reductions (R1: 3–1 d; R2: 12–7 d), increasing organic loading rates (OLR) (R1: 7.6–21.4 kg COD m−3 d−1; R2: 1.0–2.5 kg COD m−3 d−1). General chemical oxygen demand (COD) removal reached 68%, with system sensitivity to high solids, while biogas production and methane yield (MY) remained stable, reaching 264 L/d and 352 L CH4/kg COD removed. Microbial analysis identified Clostridium sensu stricto_1, Methanosarcina, and Methanoculleus as key taxa supporting process stability. These results demonstrate the potential of two-stage EGSB systems for sustainable pig manure valorisation. 2026-02-11T09:34:53Z 2026-02-11T09:34:53Z 2026-04 journal article Aragón-Barroso, A. J., Gallardo-Altamirano, M. J., Castellano-Hinojosa, A., González-Martínez, A., González-López, J., & Osorio, F. (2026). Start-up of novel pilot-scale plant based on two-stage expanded granular sludge bed reactors for pig slurry treatment: Performance and microbial dynamics. Bioresource Technology, 445, 134075. https://doi.org/10.1016/j.biortech.2026.134075 https://hdl.handle.net/10481/110863 10.1016/j.biortech.2026.134075 eng info:eu-repo/grantAgreement/EU/PRTR/TED2021-132494B http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Elsevier