<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/109965">
      <dc:title>New Method To Estimate Total Polyphenol Excretion: Comparison of Fast Blue BB versus Folin–Ciocalteu Performance in Urine</dc:title>
      <dc:creator>Hinojosa Nogueira, Daniel José</dc:creator>
      <dc:creator>Muros Molina, José Joaquín</dc:creator>
      <dc:creator>Rufián Henares, José Ángel</dc:creator>
      <dc:creator>Pastoriza de la Cueva, Silvia</dc:creator>
      <dc:subject>Polyphenols</dc:subject>
      <dc:subject>Daily intake</dc:subject>
      <dc:subject>Urinary excretion</dc:subject>
      <dc:description>This work was supported by the project AGL2014-53895-R from the Spanish Ministry of Economy and Competitiveness and the European Fund for Regional Development.</dc:description>
      <dc:description>Polyphenols are bioactive substances of vegetal origin with a significant impact on human health. The assessment of polyphenols intake and excretion is therefore important. The Folin-Ciocalteu (F-C) method is the reference assay to measure polyphenols in foods as well as their excretion in urine. However, many substances can influence the method, making it necessary to conduct a prior clean up using solid phase extraction (SPE) cartridges. In this paper we demonstrate the use of the Fast Blue BB reagent (FBBB) as a new tool to measure the excretion of polyphenols in urine. Contrary to F-C, FBBB showed no interference in urine, negating the time-consuming and costly SPE clean up. In addition, it showed excellent linearity (r2 = 0.9997) with a recovery of 96.4% and a precision of 1.86-2.11%. The FBBB method was validated to measure the excretion of polyphenols in spot urine samples from Spanish children, showing a good correlation between polyphenols intake and excretion.</dc:description>
      <dc:date>2026-01-20T12:21:16Z</dc:date>
      <dc:date>2026-01-20T12:21:16Z</dc:date>
      <dc:date>2017-05-05</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Publisher version: Hinojosa-Nogueira, D., Muros, J., Rufian-Henares, J. A., &amp; Pastoriza, S. (2017). New method to estimate total polyphenol excretion: Comparison of Fast Blue BB versus Folin–Ciocalteu performance in urine. Journal of agricultural and food chemistry, 65(20), 4216-4222. https://pubs.acs.org/doi/10.1021/acs.jafc.7b01000</dc:identifier>
      <dc:identifier>0021-8561</dc:identifier>
      <dc:identifier>1520-5118</dc:identifier>
      <dc:identifier>https://hdl.handle.net/10481/109965</dc:identifier>
      <dc:identifier>10.1021/acs.jafc.7b01000</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by-nc-nd/4.0/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Attribution-NonCommercial-NoDerivatives 4.0 Internacional</dc:rights>
      <dc:publisher>American Chemical Society</dc:publisher>
   </ow:Publication>
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