Fructooligosaccharides exert intestinal anti-inflammatory activity in the CD4+ CD62L+ T cell transfer model of colitis in C57BL/6J mice Capitán-Cañadas, Fermín Ocón, Borja Aranda Clemente, Carlos José Anzola, Andrea Suárez Ortega, María Dolores Zarzuelo Zurita, Antonio Sánchez De Medina López-Huertas, Fermín Martínez Augustín, María Olga Fructooligosaccharides Prebiotic Colitis This work was funded by Fundación Ramón Areces, by the Ministerio de Economía y Competividad (SAF2008-01432, AGL2008-04332, SAF2011-22922 and SAF2011-22812), and by Junta de Andalucía (CTS164 and CTS6736). BO and CJA are funded by Ministery of Education. AA was funded by Junta de Andalucía. CIBERehd (Centro de Investigación Biomédica en Red, Enfermedades Hepáticas y Digestivas) is funded by the Instituto de Salud Carlos III. Purpose Fructooligosaccharides (FOS) are used as functional foods due to their prebiotic effects. Intestinal anti-inflammatory activity has been established in most, but not all, studies in animal models of colitis, using mainly chemically induced inflammation. Our goal was to test the effect of FOS (degree of polymerization 2–8) in the chronic, lymphocyte-driven CD4+ CD62L+ T cell transfer model of colitis. Methods Colitis was induced by transfer of CD4+ CD62L+ T cells to C57BL/6J Rag1−/− mice. FOS (75 mg day−1) was administered by gavage as a post-treatment. Three groups were established: non-colitic (NC), colitic control (C, CD4+ CD62L+ transferred mice treated with vehicle) and colitic+FOS (C+FOS, similar but treated with FOS). Mice were killed after 13 days. Results Treatment of mice with FOS ameliorated colitis, as evidenced by an increase in body weight, a lesser myeloperoxidase and alkaline phosphatase activities, a lower secretion of proinflammatory cytokines by mesenteric lymph node cells ex vivo (IFN-γ, IL-17, and TNF-α), and a higher colonic expression of occludin (C+FOS vs. C, p < 0.05). Increased relative abundance of lactic acid bacteria was observed in FOS-treated mice (p < 0.05). Conclusions FOS exert intestinal anti-inflammatory activity in T lymphocyte-dependent colitis, suggesting it may be useful in the management of inflammatory bowel disease in appropriate conditions. 2026-01-16T07:36:47Z 2026-01-16T07:36:47Z 2015 journal article Published version: Capitán-Cañadas, F., Ocón, B., Aranda, C.J. et al. Fructooligosaccharides exert intestinal anti-inflammatory activity in the CD4+ CD62L+ T cell transfer model of colitis in C57BL/6J mice. Eur J Nutr 55, 1445–1454 (2016). https://doi.org/10.1007/s00394-015-0962-6 https://hdl.handle.net/10481/109768 10.1007/s00394-015-0962-6 eng open access Springer Nature