Quantitative proteomic analysis of compartmentalized signaling networks Hernández-Valladares, María Aran, Verónica Prior, Ian A. RAS Isogenic cells SILAC proteomics The authors thank J. R. Wis´niewski, G. Palmisano, and T. Geiger for sharing further details of their phosphoproteomic protocols. Our research is funded by North West Cancer Research, the BBSRC (BB/G018162), and the Wellcome Trust (WT085201). Ras proteins operate predominantly from the plasma membrane; however, they have also been localized to most intracellular compartments. Various functions and signaling outputs have been ascribed to endomembranous Ras although systematic comparison and measurement of potential outputs have not yet been carried out. We describe the methodology for isolating and measuring compartment-specific signaling networks using quantitative proteomics. This approach reveals the potential of a subcellular platform for supporting specific outputs and will inform subsequent studies of endogenous isoform-specific Ras signaling. 2026-01-15T13:00:28Z 2026-01-15T13:00:28Z 2014 journal article Hernández-Valladares, M.; Aran, V. y Prior, I. A. (2014). Quantitative proteomic analysis of compartmentalized signaling networks. Methods in Enzymology Volume 535, 2014, Pages 309-325. https://doi.org/10.1016/B978-0-12-397925-4.00018-3 https://hdl.handle.net/10481/109758 10.1016/B978-0-12-397925-4.00018-3 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier