Relationship between plasma S-Klotho and cardiometabolic risk in sedentary adults Amaro Gahete, Francisco José Jurado Fasoli, Lucas Sánchez Delgado, Guillermo García Lario, José Vicente Castillo Aguayo, Manuel José Ruiz Ruiz, Jonatan Aging Biomarker Cholesterol This study was funded by the Spanish Ministry of Economy and Competitiveness via the Fondo de Investigación Sanitaria del Instituto de Salud Carlos III (PI13/01393), Retos de la Sociedad (DEP2016-79512-R) and European Regional Development Funds (ERDF), the Spanish Ministry of Education (FPU14/04172), the Spanish Ministry of Education and Science (Red EXERNET DEP2005-00046), the Fundación Iberoamericana de Nutrición (FINUT), the Redes Temáticas de Investigación Cooperativa RETIC (Red SAMID RD16/0022), the AstraZeneca HealthCare Foundation, the University of Granada Plan Propio de Investigación 2016 -Excellence actions: Unit of Excellence on Exercise and Health (UCEES) - and Plan Propio de Investigación 2018 and 2019 - Programa Contratos-Puente, and the Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades (ERDF: ref. SOMM17/6107/UGR). This cross-sectional study investigates the relationship between the shed form of the Klotho protein (S-Klotho) in plasma, and cardiometabolic risk in healthy, sedentary adults. The study subjects were 214 healthy, sedentary adults (~64% women). Data were collected during the baseline assessments of two randomized controlled trials: The FIT-AGEING study (n=74 [~50% women] middle-aged adults aged 40-65 years) and the ACTIBATE study (n=140 [~70% women] young adults aged 18-25 years). A sex-specific cardiometabolic risk score was calculated for each subject based on waist circumference, systolic and diastolic blood pressure, and plasma glucose, high-density lipoprotein cholesterol and triglycerides. A significant inverse relationship was detected between S-Klotho and the cardiometabolic risk score of both the middle-aged men and women (β=-0.658, R2=0.433, P<0.001 and β=-0.442, R2=0.195, P=0.007) which persisted after adjusting for actual age, energy intake, and VO2max. No significant association was found between S-Klotho and cardiometabolic risk score for the young, healthy adults (P>0.5), nor for the young, healthy men and women when analysed separately (all P>0.1). In conclusion, in healthy, sedentary, middle-aged adults, but not in young, healthy, sedentary adults, higher plasma S-Klotho concentrations are associated with a lower cardiometabolic risk score. 2025-12-19T11:47:23Z 2025-12-19T11:47:23Z 2020-01-20 journal article Amaro-Gahete, F. J., Jurado-Fasoli, L., Sanchez-Delgado, G., García-Lario, J. V., Castillo, M. J., & Ruiz, J. R. (2020). Relationship between plasma S-Klotho and cardiometabolic risk in sedentary adults. Aging (Albany NY), 12(3), 2698. https://doi.org/10.18632/aging.102771 1945-4589 https://hdl.handle.net/10481/109026 10.18632/aging.102771 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Impact Journals