Impact of chronic exposure of rats to bisphenol A from perinatal period to adulthood on intraprostatic levels of 5α-reductase isozymes, aromatase, and genes implicated in prostate cancer development Sánchez Medina, María Pilar Castro, Beatriz Martínez Rodríguez, Sergio Ríos Pelegrina, Rosa María García Del Moral Garrido, Raimundo Torres De Pinedo, Jesús Manuel Ortega Sánchez, Esperanza Bisphenol A (BPA) 5a-reductase isozymes Aromatase Prostate cancer This research was funded by the Spanish Ministry of Science and Innovation [BFU 2008–05340] and the Andalusian Regional Government [CTS-202 Endocrinology & Metabolism Group]. Funding for open access charge: Universidad de Granada / CBUA. All procedures complied with the EC Directive 86/609/EEC for animal experiments, and the protocols were approved by the Animal Experimentation Ethics Committee of the University of Granada, Spain (Ref. 412-2012-CEEA). The synergetic effect of estrogens and androgens is known to play a crucial role in the physiopathology of the prostate gland. Bisphenol A (BPA) is an endocrine disrupting compound that can interfere with endocrine hormone functioning and thereby influence prostate development. The objective of this study was to examine the impact on prostate expression of aromatase, 5α-R isozymes, and prostate cancer-related genes of exposure to low doses of BPA from perinatal period to adulthood. Vehicle or BPA (2.5 μg/kg b.w./day) was administered to gestating Wistar rats from gestational day 12 (GD12) to parturition and then to their male pups from postnatal day 1 (PND1) until euthanization on PND90. Their prostate glands were examined by qRT-PCR, Western blot, PCR array, and morphological study. mRNA and protein levels of 5α-R2 were significantly reduced and mRNA and protein levels of aromatase were significantly increased in BPA-treated animals, which also showed modifications of 8 out of the 84 key genes implicated in the development of prostate cancer. Because BPA interferes with genes involved in intraprostatic androgen and estrogen production and others implicated in prostate cancer, research is warranted into the prostate disease risk associated with chronic low-dose BPA exposure throughout life. 2025-12-16T13:22:50Z 2025-12-16T13:22:50Z 2022-09 journal article Sánchez P, Castro B, Martínez-Rodríguez S, Ríos-Pelegrina R, Del Moral RG, Torres JM, Ortega E. Impact of chronic exposure of rats to bisphenol A from perinatal period to adulthood on intraprostatic levels of 5α-reductase isozymes, aromatase, and genes implicated in prostate cancer development. Environ Res. 2022 Sep;212(Pt A):113142. doi: 10.1016/j.envres.2022.113142. Epub 2022 Apr 1. 1096-0953 0013-9351 https://hdl.handle.net/10481/108875 10.1016/j.envres.2022.113142 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier