Pea protein’s interfacial behavior and emulsifying capacity as affected by high-pressure homogenization treatments: an in-depth study with dilatational rheology characterization D’Alessio, Giulia Maldonado Valderrama, Julia del Castillo-Santaella, Teresa Sabatucci, Annalaura Francioso, Antonio Pittia, Paola Di Mattia, Carla Daniela Dilational rheology Emulsions Interfacial layer This study evaluated the effects of high-pressure homogenization (HPH) at 60 and 100 MPa (5 cycles) on the interfacial properties and emulsifying activity of pea proteins (PP, PP60, and PP100). Pendant drop analysis showed that HPH treatments decreased the interfacial activity of pea proteins, altering the viscoelasticity of the adsorbed layer in a frequency-dependent manner. Structural analysis revealed that HPH promoted protein aggregation, mainly affecting legumins, which was directly linked to weaker interfacial interactions. As a result, emulsions stabilized with treated proteins (EP60 and EP100) exhibited immediate destabilization phenomena, such as flocculation and creaming, as confirmed by droplet size distribution, backscattering profiles, and Turbiscan® Stability Index measurements. The HPH-processing conditions adopted in this study impaired the emulsifying performance of pea proteins. These findings highlight the importance of selecting appropriate processing conditions to balance protein functionality. Future research should explore alternative pressure/cycle combinations to unveil the optimized conditions to induce beneficial structural changes while minimizing aggregation/oligomerization. Overall, this work provides insights into how HPH affects pea protein structure, interfacial behavior, and emulsion stability, supporting the design of more stable plant-based emulsions. 2025-12-05T11:53:26Z 2025-12-05T11:53:26Z 2025-11 journal article D’Alessio, G., Maldonado-Valderrama, J., Castillo-Santaella, T. del, Sabatucci, A., Francioso, A., Pittia, P., & Di Mattia, C. D. (2025). Pea protein’s interfacial behavior and emulsifying capacity as affected by high-pressure homogenization treatments: an in-depth study with dilatational rheology characterization. Food Hydrocolloids, 167(111427), 111427. https://doi.org/10.1016/j.foodhyd.2025.111427 https://hdl.handle.net/10481/108624 10.1016/j.foodhyd.2025.111427 eng info:eu-repo/grantAgreement/EU/NextGenerationEU/ECS00000041 http://creativecommons.org/licenses/by/4.0/ open access Atribución 4.0 Internacional Elsevier