Amyloid-β and Bacterial Lipopolysaccharide at Implants With Peri-Implantitis: Ex Vivo Colocalization and Decontamination Protocol Toledano Osorio, Manuel Cifuentes-Jiménez, Carolina Toledano Pérez, Manuel Sanz, Mariano Osorio Ruiz, Raquel Amyloid-β ATR-FTIR Lipopolysaccharide peri-implantitis Sodium hypochlorite Titanium This work was supported by Agencia Estatal de Investigación and Ministerio de Ciencia e Innovación. Grants PID2020-114694RB-I00 and PID2023-151623OB-I00 funded by MCIN/AEI 10.13039/501100011033. Authors want to thank Klockner S.A. (Madrid, Spain) for providing the titanium discs. Aim To study the differential presence of amyloid-β and bacterial lipopolysaccharide (LPS) in freshly extracted titanium implants, either affected by peri-implantitis (PI) or explanted by other causes, and to address a method for removal LPS and amyloid-β from contaminated surfaces. Methods Twenty-four explanted implants were harvested from patients with (n = 12) or without (n = 12) peri-implantitis, and their surfaces were analyzed by attenuated total reflectance (ATR) and Fourier transform infrared spectroscopy (FTIR) to localize amyloid-β and LPS. Presence of amyloid-β on the implants surfaces was further analyzed by light microscopy after specific amyloid staining with Congo red. Titanium discs were contaminated with LPS and amyloid-β, these discs as well as six contaminated implants were treated with 0.25% NaOCl to assess its decontamination ability. Results LPS and amyloid-β were observed at PI affected implant surfaces, but not in implants extracted by other causes. 0.25% NaOCl application was an efficient method for removing LPS and amyloid-β from titanium surfaces. Conclusions The concurrent presence of LPS and amyloid-β on the surface of implants affected by PI was demonstrated and it may act as potential comediators of PI inflammatory process. Eliminating these products from implants surfaces is possible after a proteolytic agent (0.25% NaOCl) application. 2025-10-30T10:26:07Z 2025-10-30T10:26:07Z 2025 journal article Published version: Toledano‐Osorio, M., Cifuentes‐Jiménez, C., Toledano, M., Sanz, M., & Raquel, O. Amyloid‐β and Bacterial Lipopolysaccharide at Implants With Peri‐Implantitis: Ex Vivo Colocalization and Decontamination Protocol. Journal of Periodontal Research, 17 February 2025. https://doi.org/10.1111/jre.13386 https://hdl.handle.net/10481/107589 10.1111/jre.13386 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ embargoed access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wiley online library