Optimization of extraction method to obtain a phenolic compounds-rich extract from Moringa oleifera Lam leaves Rodríguez-Pérez, Celia Quirantes-Piné, Rosa Fernández-Gutiérrez, Alberto Segura Carretero, Antonio Maceration Ultrasound-assisted extraction Folin–Ciocalteu Bioactive compounds HPLC–ESI–QTOF–MS Moringa oleifera Lam is considered one of the most useful tree in the world because every part of the Moringa tree can be used such as nutritional supplement, for medication, and industrial purposes. Conventional solid–liquid extraction and ultrasound-assisted extraction (UAE) were performed using different solvents and mixtures of solvents with water. The total phenolic content was determined using Folin–Ciocalteu assay. UAE using ethanol:water (50:50) was the best extraction procedure, which allowed 47 ± 4 mg gallic acid equivalents (GAE)/g dry leaf to be obtained. In addition, high-performance liquid chromatography coupled to electrospray ionization quadropole-time of flight mass spectrometry (HPLC–ESI–QTOF–MS) was used to characterize the bioactive compounds in the resulting extract. Consequently, 59 compounds were tentatively characterized, phenolic acid derivatives and flavonoids being the most abundant. Furthermore, 30 of these compounds were tentatively identified for the first time in M. oleifera leaves. This study shows that leaves from M. oleifera are a good nutritional resource used as a nutritional supplement and may carry additional opportunities for food ingredient innovations, pharmaceutical and cosmetics products. 2025-07-25T10:29:39Z 2025-07-25T10:29:39Z 2015-04 journal article Industrial Crops and Products Volume 66, 2015, Pages 246-254 https://hdl.handle.net/10481/105669 https://doi.org/10.1016/j.indcrop.2015.01.002 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier