<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/105660">
      <dc:title>HPLC–ESI–QTOF–MS as a Powerful Analytical Tool for Characterising Phenolic Compounds in Olive-leaf Extracts</dc:title>
      <dc:creator>Quirantes-Piné, Rosa</dc:creator>
      <dc:creator>Lozano-Sánchez, Jesús</dc:creator>
      <dc:creator>Herrero, Miguel</dc:creator>
      <dc:creator>Ibáñez, Elena</dc:creator>
      <dc:creator>Segura Carretero, Antonio</dc:creator>
      <dc:creator>Fernández-Gutiérrez, Alberto</dc:creator>
      <dc:subject>Electrospray ionisation</dc:subject>
      <dc:subject>High-performance liquid chromatography</dc:subject>
      <dc:subject>Quadrupole time-of-ﬂight</dc:subject>
      <dc:subject>Olive leaves</dc:subject>
      <dc:subject>Phenolic compounds</dc:subject>
      <dc:description>Introduction: Olea europaea L. leaves may be considered a cheap, easily available natural source of phenolic compounds. In a previous study we evaluated the possibility of obtaining bioactive phenolic compounds from olive leaves by pressurised liquid extraction (PLE) for their use as natural anti-oxidants. The alimentary use of these kinds of extract makes comprehensive knowledge of their composition essential.&#xd;
Objective: To undertake a comprehensive characterisation of two olive-leaf extracts obtained by PLE using high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS).&#xd;
Method: Olive leaves were extracted by PLE using ethanol and water as extraction solvents at 150°C and 200°C respectively. Separation was carried out in a HPLC system equipped with a C₁₈-column working in a gradient elution programme coupled to ESI-QTOF-MS operating in negative ion mode.&#xd;
Results: This analytical platform was able to detect 48 compounds and tentatively identify 31 different phenolic compounds in these extracts, including secoiridoids, simple phenols, flavonoids, cinnamic-acid derivatives and benzoic acids. Lucidumoside C was also identified for the first time in olive leaves.&#xd;
Conclusion: The coupling of HPLC-ESI-QTOF-MS led to the in-depth characterisation of the olive-leaf extracts on the basis of mass accuracy, true isotopic pattern and tandem mass spectrometry (MS/MS) spectra. We may conclude therefore that this analytical tool is very valuable in the study of phenolic compounds in plant matrices.</dc:description>
      <dc:date>2025-07-25T09:32:26Z</dc:date>
      <dc:date>2025-07-25T09:32:26Z</dc:date>
      <dc:date>2012-09-15</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Phytochemical Analysis 2013, 24(3): 213-223</dc:identifier>
      <dc:identifier>https://hdl.handle.net/10481/105660</dc:identifier>
      <dc:identifier>10.1002/pca.2401</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by-nc-nd/4.0/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Attribution-NonCommercial-NoDerivatives 4.0 Internacional</dc:rights>
      <dc:publisher>Wiley</dc:publisher>
   </ow:Publication>
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