Embryo secretome in predicting embryo quality and IVF treatment outcome Toporcerová, Silvia Badovská, Zuzana Kriváková, Eva Mikulová, Viktória Mareková, Mária Altmäe, Signe Rabajdová, Miroslava Assisted reproductive technology Biomarker Embryo The article processing charge was funded by Slovak Research and Development Agency: Non-invasive diagnostics based on ncRNA/(APVV-22-0357) and the Ministry of Education, Research, Development and Youth of the Slovak Republic: The effect of hyaluronic acid on endometrial receptivity in the IVF process (1/0747/24). SA is supported by projects Endo-Map PID2021-12728OB-I00 and ROSY CNS2022-135999 funded by MICIU/AEI/10.13039/501100011033 and by FEDER. In IVF treatment, accurate prediction of embryo quality and successful embryo implantation are critical challenges. Recent research has highlighted the importance of the embryo secretome, the collection of molecules secreted by the embryo into the culture medium, in assessing embryo quality as a non-invasive preimplantation testing platform. The secretome plays a role in many essential processes, from gamete maturation to embryonic development. Molecules such as cell-free DNA, mitochondrial DNA and small non-coding RNA, including microRNA and PIWI-interacting RNA, together with the proteome, metabolome, microbiome and extracellular vesicles, have emerged as important players in predicting IVF outcomes. These molecules, present in the culture medium, have shown correlations with embryo viability, ploidy and implantation potential. Nevertheless, extensive validation in larger cohorts and an assessment of the applicability of the identified biomarkers in clinical settings is warranted. This article summarizes the molecular markers analysed in spent embryo culture medium and their potential for assessing embryo quality and predicting success in IVF outcomes. 2025-07-02T09:41:42Z 2025-07-02T09:41:42Z 2025-07 journal article Toporcerová, Silvia et al. Reproductive BioMedicine Online Volume 51, Issue 1, July 2025, 104825. https://doi.org/10.1016/j.rbmo.2025.104825 https://hdl.handle.net/10481/105036 10.1016/j.rbmo.2025.104825 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier