Dual optically responsive paper-membrane for simple, portable, and versatile control of total and volatile acidity in wines López Aveiga, Melany G. Fernández Ramos, María Dolores Martos Núñez, María Vanesa González Casado, Antonio Medina Castillo, Antonio Luis This work has been supported by Grant [C. EXP. 030. UGR23 and C. EXP. 103. UGR23 funded by Consejería de Universidad, Investigación e Innovación and by ERDF Andalusia Program 2021–2027, and the Project European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie-RISE Grant Agreement “SUSTAINABLE” No. 101007702 (https://www.projectsustainable.eu/). Funding for open access charge: Universidad de Granada/CBUA. The authors are grateful for the technical support and advice provided by the collaborating companies DNA phone (Italy: https://www.dnaphone.it/) and Cloner (Spain: https://www.cloner.es/index-5.html). Antonio L. Medina-Castillo. Acknowledges funding by Plan Propio-investigación y Transferencia de la Universidad de Granada: “Programa 9. Proyectos de investigación para la incorporación de jóvenes Doctores a Nuevas Líneas de investigación en Grupos de la UGR”. Background: The high consumption of expensive and polluting reagents and the use of tedious reactions with long analysis times are key problems in the quantification of total (TA) and volatile acidity (VA) in wine by official methods (titration with sodium hydroxide and enzymatic method). Results: We have exploited the advantages and versatility of our optically responsive paper-membrane (PaperFM) to perform the dual simultaneous determination (fluorescent and colorimetric) of TA and VA in wine. The technology was tested by performing the simultaneous double quantification (fluorescence and colorimetry) of TA and VA of wine samples from different origins, obtaining an excellent correlation between the results obtained by colorimetry and those obtained by fluorescence. Furthermore, the results were successfully validated using official reference methods (titration with sodium hydroxide and enzymatic method) in an accredited laboratory. Significance: We have developed the first optical sensing method that allows the quantification of the TA and VA in wine. The results presented in this work demonstrate that our optical sensing technology enables direct, simple, reversible, reusable, fast, cost-effective, and environmentally friendly simultaneous dual quantification (fluorescent and colorimetric) of TA and VA in wine samples without the use of additional reagents. 2025-04-29T11:57:00Z 2025-04-29T11:57:00Z 2025-06-22 journal article M.G. López Aveiga et al. Analytica Chimica Acta 1356 (2025) 344016. https://doi.org/10.1016/j.aca.2025.344016 https://hdl.handle.net/10481/103846 10.1016/j.aca.2025.344016 eng info:eu-repo/grantAgreement/EC/H2020/101007702 http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier