Dielectric constant of flagellin proteins measured by scanning dielectric microscopy Lozano, Helena Blanco-Cabra, Nuria Millan-Solsona, Ruben Torrents, Eduard Fumagalli, Laura Gomila, Gabriel Fabregas, Rene Dielectric Constant Flagellin Proteins Scanning Dielectric Microscopy Bacterial Flagella Nanoscale Characterization Nano-Modeling The dielectric constant of flagellin proteins in flagellar bacterial filaments ∼10–20 nm in diameter is measured using scanning dielectric microscopy. We obtained for two different bacterial species (Shewanella oneidensis MR-1 and Pseudomonas aeruginosa PAO1) similar relative dielectric constant values εSo = 4.3 ± 0.6 and εPa = 4.5 ± 0.7, respectively, despite their different structure and amino acid sequence. The present results show the applicability of scanning dielectric microscopy to nanoscale filamentous protein complexes and to general 3D macromolecular protein geometries, thus opening new avenues to study the relationship between the dielectric response and protein structure and function. 2025-02-03T08:42:56Z 2025-02-03T08:42:56Z 2018 journal article https://hdl.handle.net/10481/101841 10.1039/C8NR06190D eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional