Study of short- and long-term storage of teeth and its influence on DNA Rubio, Leticia Martínez González, Luis Javier Martínez Espín, Esther Martín De Las Heras, Stella Forensic science Forensic odontology DNA degradation short tandem repeat CSF1PO D16S539 D7S820 THO1 TPOX D3S1358 amelogenin teeth DNA degradation can interfere with the resolution of forensic cases. Allelic dropout often reduces the opportunity for adequate comparisons between degraded and reference samples. This study analyzed DNA degradation in 24 extracted teeth after storage at room temperature for 0, 2, 5, and 10 years. DNA concentration, quantified by dot-blot hybridization, declined significantly for the first 2 years, but there was no significant further degradation from the second to the tenth year of storage. COfiler™ analysis was used and the allelic dropout ratio for the amelogenin locus relative to CSF1PO locus was also estimated. Statistically significant differences were found between fresh teeth and teeth from the 2- and 5-year groups but not from the 10-year group. Under our storage conditions most of the DNA degradation occurred during the first 2 years. Further research is needed to control for individual and external factors that could affect DNA. 2025-02-03T07:50:34Z 2025-02-03T07:45:42Z 2025-02-03T07:50:34Z 2025-02-03T07:45:42Z 2009-10-23 2009-10-23 journal article J Forensic Sci. 2009 Nov;54(6):1411-3 https://hdl.handle.net/10481/101814 https://hdl.handle.net/10481/101814 10.1111/j.1556-4029.2009.01159.x eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional WILEY