Conjugation of lentivirus to paramagnetic particles via non-viral proteins allows efficient concentration and infection of primary Acute Myeloid Leukaemia cells Chan, L Nesbeth, D Mackey, t Galea-Lauri, J Gaken, J Mufti, F Farzaneh, F Darling, D Collins, Mary Martín Molina, Francisco gene therapy targeting Lentiviral vectors Myeloid leukemia cells concentration Conjugation Nonviral producer cell proteins incorporated into retroviral vector surfaces profoundly influence infectivity and in vivo half-life. We report the purification and concentration of lentiviral vectors using these surface proteins as an efficient gene transduction strategy. Biotinylation of these proteins and streptavidin paramagnetic particle concentration enhances titer 400- to 2,500-fold (to 10(9) CFU/ml for vesicular stomatitis virus G protein and 5 x 10(8) for amphotropic murine leukemia virus envelope). This method also uses newly introduced membrane proteins (B7.1 and DeltaLNGFR) directed to lentiviral surfaces, allowing up to 17,000-fold concentrations. Particle conjugation of lentivirus allows facile manipulation in vitro, resulting in the transduction of 48 to 94% of human acute myeloid leukemia blasts. 2025-01-31T08:33:37Z 2025-01-31T08:33:37Z 2005 journal article Journal of Virology. Volumen: 79(20); Págs: 13190-13194. (2005): https://hdl.handle.net/10481/101491 10.1128/JVI.79.20.13190-13194.2005 eng open access American Society of Microbiology