Reverse transcriptase-like activity in Trypanosoma cruzi González, Clara thomas, Maria del Carmen Martín Molina, Francisco Alcami, j Alonso, Carlos Lopez, Manuel Carlos Nuclease activity Trypanosoma cruzi LINEs The use of specific synthetic RNA homopolymers as templates and short oligonucleotides as primers has allowed evidence of the existence of a reverse transcriptase-like activity in Trypanosoma cruzi, to be revealed. The RNA:DNA products derived from this reaction are of approximately 110 nucleotides in length. The enzyme has greater affinity for poly(rA)/oligo(dT) templates than for poly(rC)/oligo(dG) having a 20 mM Mg++ ion requirement. The detected reverse transcriptase-like activity is not affected by aphidicolin and ddTTP but is inhibited by actinomycin D, novobiocin, rifamycin SV and AZT. 2025-01-31T08:13:44Z 2025-01-31T08:13:44Z 1997 journal article Acta tropica: Vol 63 Páginas 117-126 (1997) https://hdl.handle.net/10481/101461 10.1016/s0001-706x(96)00611-0 eng open access ScienceDirect - Elsevier