Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3'-end of a poly(A)+ transcript Requena, José María Martín Molina, Francisco Manuel, Soto López, Manuel Carlos Alonso, Carlos short interspersed reiterated DNA sequence Trypanosoma cruzi poly(A)+ transcript We have carried out the molecular characterization of a highly repeated DNA element, called E12, from Trypanosoma cruzi, which has been found to be interspersed along its genome. The E12 element, repeated about 5.6 × 103 times, is found in most of the chromosomal bands of the parasite. Three subregions may be defined within the element on the basis of sequence similarities with other trypanosome genomic sequences. Northern blot analysis demonstrated that sequences of the E12 element are present in several polyadenylated RNA species of T. cruzi. The isolation and characterization of a cDNA clone, pSPFM55, which showed hybridization with the E12 probe, indicated that only one of the E12 subregions, E12A, is found in the cDNA and that it is located at the 3'-end providing the site of polyadenylation addition. The location and high degree of nucleotide conservation of E12A suggest a possible functional role of this sequence in gene expression. 2025-01-31T07:39:02Z 2025-01-31T07:39:02Z 1994-08-02 journal article Requena, José María et al. Gene Volume 146, Issue 2, 2 September 1994, Pages 245-250. https://doi.org/10.1016/0378-1119(94)90300-X https://hdl.handle.net/10481/101414 10.1016/0378-1119(94)90300-X eng open access Elsevier