Cytoplasmic nuclear translocation of the HSP70 protein during enviromental stress in Trypanosoma cruzi Martín Molina, Francisco Requena, José María Alonso, Carlos López, Manuel Carlos The present study provides immunological evidence of the constitutive presence of the Hsp70 protein in the cytoplasm of logarithmically growing parasites cultured at the normal temperature of 28°C and of the translocation of the protein to the nucleus upon a heat shock treatment (2 hours at 37°C). The nuclear translocation of the protein must depend on other factors beside the temperature per se since at 28°C, in stationary phase growing parasites, the Hsp70 protein was present in both the cytoplasm and the nucleus. During recovery at 28°C the protein leaves the nuclei but the nuclear-cytoplasmic translocation of the protein is a much more gradual process than its initial transport to the nucleus. Since the isoform of the nuclear Hsp70 is different from that found in the cytoplasm it is likely that before translocation to the nucleus the cytoplasmic Hsp70 nuclear precursor must undergo a specific modification 2025-01-30T13:15:09Z 2025-01-30T13:15:09Z 1993 journal article Biochemical Biophysical Research Communications V: 146 Pág 245-250 Fecha 1993 https://hdl.handle.net/10481/101372 https://doi.org/10.1006/bbrc.1993.2372 eng open access Elsevier