Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs Di Mauro, Vittoria Iafisco, Michele Salvarini, Nicolo Vacchiano, Marco Carullo, Pierluigi Ramírez-Rodríguez, Gloria Belén Patricio, Tatiana Tampieri, Anna Miragoli, Michele Catalucci, Daniele Calcium phosphate microRNA Nanomedicine Cardiomyocytes Drug-delivery Aim: To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart. Materials & methods: CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated. Results: CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo. Conclusion: CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes. 2025-01-30T11:37:49Z 2025-01-30T11:37:49Z 2016 journal article 43. Di Mauro V, Iafisco M, Salvarani N, Vacchiano Marco, Ramírez-Rodríguez G.B., Patricio T, et al. (2016) Bio-inspired negatively-charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs. Nanomedicine. 11-8 https://hdl.handle.net/10481/101315 10.2217/nnm.16.26 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional