miR-410 controls adult SVZ neurogenesis by targeting neurogenic genes Morell Hita, María Over-expression of the early neural inducer, Noggin, in nestin positive subventricular zone (SVZ), neural stem cells (NSC) promotesproliferation and neuronal differentiation of neural progenitors and inhibits theexpression ofaCNS-enrichedmicroRNA-410(miR-410)(Morelletal.,2015).Whenexpressedinneurospheresderivedfrom the adultSVZ, miR-410inhibits neuronal andoligodendrocyte differentiation, and promotes astrocyte differenti ation. miR-410 also reverses the increase in neuronal differentiation and decreased astroglial differentiation caused by Noggin over-expression. Conversely, inhibition of miR-410 activity promotes neuronal and decreases astroglial differentiation of NSC. Using computer prediction algorithms and luciferase reporter assays we identi f ied multiple neurogenic genes including Elavl4 as downstream targets of miR-410 via the canonical miRNA-3′ UTR interaction. Over-expression of Elavl4 transcripts without the endogenous 3′UTR rescued the decrease in neuronal differentiation caused by miR-410 overexpression. Interestingly, we also observedthat miR-410 affect edneurite morphology;over-expressionof miR-410resultedintheformation ofshort,unbranchedneurites. We conclude that miR-410 expression provides a new link between BMP signaling and the crucial lineage choice of adult neural stem cells via its ability to bind and control the expression of neurogenic gene transcripts. 2025-01-30T10:49:44Z 2025-01-30T10:49:44Z 2016 journal article Tsan YC, Morell MH, O'Shea KS. miR-410 controls adult SVZ neurogenesis by targeting neurogenic genes. Stem Cell Res. 2016 Sep;17(2):238-247. doi: 10.1016/j.scr.2016.07.003. Epub 2016 Jul 17. PMID: 27591480. https://hdl.handle.net/10481/101261 10.1016/j.scr.2016.07.003 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Stem Cell Research