L1 retrotransposition in human neural progenitor cells Morell Hita, María Long Interspersed Element-1 (LINE-1 or L1) retrotransposons have dramatically impacted the human genome. L1s must retrotranspose in the germ-line or during early development to ensure their evolutionary success; yet the extent to which this process impacts somatic cells is poorly understood. We previously demonstrated that engineered human L1s can retrotranspose in adult rat hippocampus progenitor cells (NPCs) in vitro and in the mouse brain in vivo1. Here, we demonstrate that NPCs isolated from human fetal brain and NPCs derived from human embryonic stem cells (hESCs) support the retrotransposition of engineered human L1s in vitro. Furthermore, we developed a quantitative multiplex polymerase chain reaction that detected an increase in the copy number of endogenous L1s in the hippocampus and in several regions of adult human brains when compared to the copy number of endogenous L1s in heart or liver genomic DNAs from the same donor. These data suggest that de novo L1 retrotransposition events may occur in the human brain and, in principle, have the potential to contribute to individual somatic mosaicism. 2025-01-30T10:44:07Z 2025-01-30T10:44:07Z 2009-11-27 journal article Coufal NG, Garcia-Perez JL, Peng GE, Yeo GW, Mu Y, Lovci MT, Morell M, O'Shea KS, Moran JV, Gage FH. L1 retrotransposition in human neural progenitor cells. Nature. 2009 Aug 27;460(7259):1127-31. doi: 10.1038/nature08248. Epub 2009 Aug 5. PMID: 19657334; PMCID: PMC2909034. https://hdl.handle.net/10481/101257 doi: 10.1038/nature08248. Epub 2009 Aug 5. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional