Reliable transfection of Plasmodium falciparum using non-commercial plasmid mini preparations Hernandez Valladares, Maria Plasmodium falciparum transfection plasmid preparation Transfection of Plasmodium falciparum is instrumental in the research on this parasite. However, the actual transfection protocol has not changed significantly since the first description and it is generally believed that large amounts of highly pure plasmid DNA are needed for successful transfection. Here, we report the transfection of P. falciparum using a protocol based on non-commercial mini-preparations of plasmid DNA. This method permits the reliable transfection of P. falciparum using less resources and lower costs, with a success rate comparable with currently used methods. A moderate throughput may be achieved using this method, providing a first step towards systematic transfection approaches in this parasite. 2025-01-30T10:29:03Z 2025-01-30T10:29:03Z 2006-07-28 journal article Spielmann, T., Dixon, M. W., Hernandez-Valladares, M., Hannemann, M., Trenholme, K. R., & Gardiner, D. L. (2006). Reliable transfection of Plasmodium falciparum using non-commercial plasmid mini preparations. International journal for parasitology, 36(12), 1245–1248. https://doi.org/10.1016/j.ijpara.2006.06.017 https://hdl.handle.net/10481/101233 10.1016/j.ijpara.2006.06.017 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional Elsevier