New perspectives on the synthesis and antichagasic activity of 3-alkoxy-1-alkyl-5-nitroindazoles Muro, Beatriz Reviriego, Felipe Navarro, Pilar Marín Sánchez, Clotilde Ramírez-Macías, Inmaculada Rosales Lombardo, María José Sánchez Moreno, Manuel Arán, Vicente J The synthesis and antiprotozoal activity of some 3-alkoxy-1-alkyl- (1, 4) and 3-alkoxy-1-(ω-aminoalkyl)-5-nitroindazoles (2, 3, 5-8) against different morphological forms of Trypanosoma cruzi are reported. These compounds were prepared using simple alkylation reactions and, usually, taking advantage of the reactivity of some indazole-derived betaines previously studied by us. Most indazole derivatives showed in vitro activities similar or higher than those of the reference drug benznidazole; this fact, along with low unspecific cytotoxicities against Vero cells shown by some of them, led to very good selectivity indexes (SI). The high efficiency of 5-nitroindazoles 1 and 2 against T. cruzi was confirmed by further in vitro studies on infection rates and by an additional in vivo study in a murine model of acute and chronic Chagas disease. Complementary analyses of the changes in the metabolites excreted by the parasite and on the ultrastructural alterations induced after treatment with indazole derivatives 1 and 2 were also conducted. 2025-01-30T09:01:04Z 2025-01-30T09:01:04Z 2014 journal article Eur J Med Chem . 2014 Mar 3:74:124-34. doi: 10.1016/j.ejmech.2013.12.025 https://hdl.handle.net/10481/101094 10.1016/j.ejmech.2013.12.025 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ embargoed access Attribution-NonCommercial-NoDerivatives 4.0 Internacional