Anisakis simplex s.l.: Larvicidal activity of various monoterpenic derivatives of natural origin against L3 larvae in vitro and in vivo Navarro, M.C. Noguera, M.A. Romero, M.C. Montilla, M.P. González de Selga, J.M. Valero, A Anisakis simplex s.l. Larvicidal activity Monoterpenic derivatives α-Pinene Ocimene nutrition education This paper describes the activity against Anisakis simplex s.l. L3 larvae of six monoterpenic derivatives obtained from different essential oils, (α-pinene, β-pinene, ocimene, myrcene, geranyl acetate, and cineole). In in vitro assays, α-pinene, ocimene and cineole showed high activity at a concentration of 125 μg/mL (48 h) but only α-pinene and ocimene were active at 62.5 μg/mL. In in vivo assays, L3 larvae and study compounds were simultaneously administered per os to Wistar rats. The most active compound was α-pinene, finding lesions in only 20% of treated rats versus 98% of controls. Further in vivo studies are required to investigate whether addition of these compounds to food could have a prophylactic effect, reducing the pathogenicity of A. simplex s.l. L3 in humans, and to explore any possible synergy among compounds. 2025-01-30T08:53:24Z 2025-01-30T08:53:24Z 2008-07-31 journal article M.C. Navarro, M.A. Noguera, M.C. Romero, M.P. Montilla, J.M. González de Selgas, A. Valero, Anisakis simplex s.l.: Larvicidal activity of various monoterpenic derivatives of natural origin against L3 larvae in vitro and in vivo, Experimental Parasitology, Volume 120, Issue 4, 2008, Pages 295-299, https://hdl.handle.net/10481/101086 https://doi.org/10.1016/j.exppara.2008.07.014 eng open access Experimental Parasitology