Peripheral inflammation-induced changes in songbird brain gene expression: 3' mRNA transcriptomic approach Kuttiyarthu Veetil, Nithya Cedraz de Oliveira, Haniel Gómez Samblás, María Mercedes Divin, Daniel Melepat, Balraj Voukali, Eleni Swiderska, Zuzana Krajzingrová, Tereza Tesicky, Martin Jung, Ferris Benes, Vladimir Madsen, Ole Vinkler, Michal Avian cytokine Differential gene expression Neurogenic inflammation Neuroimmune interaction Peripheral immunity Transcriptome Species-specific neural inflammation can be induced by profound immune signalling from periphery to brain. Recent advances in transcriptomics offer cost-effective approaches to study this regulation. In a population of captive zebra finch (Taeniopygia guttata), we compare the differential gene expression patterns in lipopolysaccharide (LPS)-triggered peripheral inflammation revealed by RNA-seq and QuantSeq. The RNA-seq approach identified more differentially expressed genes but failed to detect any inflammatory markers. In contrast, QuantSeq results identified specific expression changes in the genes regulating inflammation. Next, we adopted QuantSeq to relate peripheral and brain transcriptomes. We identified subtle changes in the brain gene expression during the peripheral inflammation (e.g. up-regulation in AVD-like and ACOD1 expression) and detected co-structure between the peripheral and brain inflammation. Our results suggest benefits of the 3'end transcriptomics for association studies between peripheral and neural inflammation in genetically heterogeneous models and identify potential targets for the future brain research in birds. 2025-01-29T08:17:16Z 2025-01-29T08:17:16Z 2023-11-25 journal article Kuttiyarthu Veetil N, Cedraz de Oliveira H, Gomez-Samblas M, Divín D, Melepat B, Voukali E, Świderská Z, Krajzingrová T, Těšický M, Jung F, Beneš V, Madsen O, Vinkler M. Peripheral inflammation-induced changes in songbird brain gene expression: 3' mRNA transcriptomic approach. Dev Comp Immunol. 2024 Feb;151:105106. doi: 10.1016/j.dci.2023.105106 https://hdl.handle.net/10481/100877 10.1016/j.dci.2023.105106 eng Grant Schemes at Charles University grant nos. GAUK 646119, PRIMUS/17/SCI/12 START/SCI/113 the Czech Science Foundation Grant No. P502/19-20152Y http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 Internacional