SOCS3 silencing attenuates eosinophil functions in asthma patients Zafra Martín, María Paz Cañas, Jose A. Mazzeo, Carla Gámez, Cristina Sanz, Verónica Fernandez-Nieto, Mar Quirce, Santiago Barranco, Pilar Ruiz Hornillos, Javier Sastre, Joaquín Del Pozo, Victoria short interfering RNAs (siRNAs) SOCS3 eosinophils asthma Eosinophils are one of the key inflammatory cells in asthma. Eosinophils can exert a wide variety of actions through expression and secretion of multiple molecules. Previously, we have demonstrated that eosinophils purified from peripheral blood from asthma patients express high levels of suppressor of cytokine signaling 3 (SOCS3). In this article, SOCS3 gene silencing in eosinophils from asthmatics has been carried out to achieve a better understanding of the suppressor function in eosinophils. SOCS3 siRNA treatment drastically reduced SOCS3 expression in eosinophils, leading to an inhibition of the regulatory transcription factors GATA-3 and FoxP3, also interleukin (IL)-10; in turn, an increased STAT3 phosphorilation was observed. Moreover, SOCS3 abrogation in eosinophils produced impaired migration, adhesion and degranulation. Therefore, SOCS3 might be regarded as an important regulator implicated in eosinophil mobilization from the bone marrow to the lungs during the asthmatic process. 2025-01-29T07:55:15Z 2025-01-29T07:55:15Z 2015 journal article Zafra MP, Cañas JA, Mazzeo C, Gámez C, Sanz V, Fernández-Nieto M, Quirce S, Barranco P, Ruiz-Hornillos J, Sastre J, del Pozo V. SOCS3 silencing attenuates eosinophil functions in asthma patients. Int J Mol Sci. 2015 Mar 10;16(3):5434-51. PMID: 25764157 https://hdl.handle.net/10481/100866 10.3390/ijms16035434 eng open access MDPI AG