The p12 subunit of human polymerase δ uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA)
Identificadores
URI: https://hdl.handle.net/10481/99993Metadatos
Mostrar el registro completo del ítemAutor
Gonzalez-Magaña, Amaia; Ibáñez De Opakua, Alain; Romano-Moreno, Miguel; Murciano Calles, Javier; Merino, Nekane; Luque Fernández, Irene; Rojas, Adriana L.; Onesti, Silvia; Blanco, Francisco J.; De Biasio, AlfredoEditorial
Elsevier
Materia
DNA polymerase DNA replication PCNA interacting sequence PIP-box Processivity Proliferating cell nuclear antigen (PCNA) Protein structure Protein-protein interaction
Fecha
2019-03-15Referencia bibliográfica
Gonzalez-Magaña A, Ibáñez de Opakua A, Romano-Moreno M, Murciano-Calles J, Merino N, Luque I, Rojas AL, Onesti S, Blanco FJ, De Biasio A. The p12 subunit of human polymerase δ uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA). J Biol Chem. 2019 Mar 15;294(11):3947-3956. doi: 10.1074/jbc.RA118.006391. Epub 2019 Jan 17. PMID: 30655288; PMCID: PMC6422072.
Patrocinador
This work was supported by the Italian Association for Cancer Research (iCARE fellowship from AIRC and the European Commission to A. D. B. and AIRC Grant IG14718 to S. O.), by Grant CTQ2017-83810-R (MINECO/FEDER, UE; to F. J. B.), by MINECO Fellowship BES-2015-075847 (to A. G.-M.), and by Basque Government Predoctoral Fellowship PRE_2016_2_0249 (to M. R.-M.)Resumen
Human DNA polymerase δ is essential for DNA replication and acts in conjunction with the processivity factor proliferating cell nuclear antigen (PCNA). In addition to its catalytic subunit (p125), pol δ comprises three regulatory subunits (p50, p68, and p12). PCNA interacts with all of these subunits, but only the interaction with p68 has been structurally characterized. Here, we report solution NMR-, isothermal calorimetry-, and X-ray crystallography-based analyses of the p12-PCNA interaction, which takes part in the modulation of the rate and fidelity of DNA synthesis by pol δ. We show that p12 binds with micromolar affinity to the classical PIP-binding pocket of PCNA via a highly atypical PIP box located at the p12 N terminus. Unlike the canonical PIP box of p68, the PIP box of p12 lacks the conserved glutamine; binds through a 2-fork plug made of an isoleucine and a tyrosine residue at +3 and +8 positions, respectively; and is stabilized by an aspartate at +6 position, which creates a network of intramolecular hydrogen bonds. These findings add to growing evidence that PCNA can bind a diverse range of protein sequences that may be broadly grouped as PIP-like motifs as has been previously suggested.