Highly inducible Tet-On vector system

Abstract

PCT/EP2012/059408 , US 15/785,388 Licenciada a Canvax Biotech en exclusividad Libertad para comercializar en todo el mundo salvo: EEUU. Libre de operar a partir del 26 de junio de 2017 Canadá, Japón, Reino Unido, Francia, Alemania, Lituania y Suiza. Libre de operar partir de 26/junio/2018 Entidad titular de derechos: FUNDACION PUBLICA ANDALUZA PROGRESO Y SALUD Nº de solicitud: EP11166754.9, EP 12727326.6, País de inscripción: España, Andalucía Fecha de registro: 19/05/2011 Nº de patente: PCT / EP2012 / 059408 Patente PCT: Sí Oficina de patentes US Fecha de publicación 21/11/2017 Número de patente 9822379 Número de solicitud 14118880

The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.