Epidemiological survey of two morphotypes of Demodex folliculorum (Prostigmata: Demodicidade) in young people from southern Spain

Abstract

Different morphotypes of Demodex from humans have been described. Among them, molecular studies have made it possible to discern between the Demodex folliculorum and Demodex brevis. Further studies showed two morphotypes of D. folliculorum harboured two different habits (human skin and human eyelashes), both of them with finger-shaped terminal opisthosoma difficult to differentiate and that can be assigned to D. folliculorum, Thus, a complete morphometric study of the species, which in this study are referred to as Demodex folliculorum species complex, was carried out. From this morphometric and meristic study two distinct morphological forms (short and long) could be identified within the Demodex folliculorum species complex. These forms differ significantly in four out of the six biometric parameters we analysed: gnathosomal length and width, podosomal width, and opisthosomal length. Moreover, a comprehensive survey of the two morphotypes from different habitats (skin and eyelashes), was carried out in young people of Southern Spain. Therefore, an analysis of 104 asymptomatic students, which were contact lens wearers, and the presence of D. folliculorum was carried out. A statistical analysis based on Bayesian zero inflated Poisson GLM has been applied to our sample data. For the age group considered (18–24 years old), the overall D. folliculorum prevalence for skin face or eye infections (at least one of them) was 19.31 % (51 people), with a statistically significant higher prevalence in males men. Furthermore, there is a slight statistical correlation between the presence of Demodex in silicone hydrogel soft contact lens wearers. This study confirms: i) the existence of two morphotypes of D. folliculorum that appear segregated due to the parasitization microhabitats,ii) a higher prevalence of mites in men than in women, iii) the existence of a high number of statistically supported double infections (skin-eyelashes). It also provides epidemiological data on the prevalence of long and short forms of D. folliculorum in a healthy young population.