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dc.contributor.authorBhojwani-Cabrera, Aysha M.
dc.contributor.authorBautista García, Alicia
dc.contributor.authorNeubrand, Veronika Elisabeth 
dc.contributor.authorMembrive Jiménez, Francisco A.
dc.contributor.authorBramini, Mattia 
dc.contributor.authorMartín-Oliva, David
dc.contributor.authorCuadros Ojeda, Miguel Ángel 
dc.contributor.authorMarín Teva, José Luis 
dc.contributor.authorNavascues Martínez, Julio 
dc.contributor.authorVangheluwe, Peter
dc.contributor.authorSepúlveda Justo, María Del Rosario 
dc.date.accessioned2025-01-08T11:23:04Z
dc.date.available2025-01-08T11:23:04Z
dc.date.issued2024-03-14
dc.identifier.citationBhojwani Cabrera, A.M. et. al. Glia. 2024;72:1201–1214. [https://doi.org/10.1002/glia.24528]es_ES
dc.identifier.urihttps://hdl.handle.net/10481/98684
dc.description.abstractMicroglia play an important protective role in the healthy nervous tissue, being able to react to a variety of stimuli that induce different intracellular cascades for specific tasks. Ca2+ signaling can modulate these pathways, and we recently reported that microglial functions depend on the endoplasmic reticulum as a Ca2+ store, which involves the Ca2+ transporter SERCA2b. Here, we investigated whether microglial functions may also rely on the Golgi, another intracellular Ca2+ store that depends on the secretory pathway Ca2+/Mn2+-transport ATPase isoform 1 (SPCA1). We found upregulation of SPCA1 upon lipopolysaccharide stimulation of microglia BV2 cells and primary microglia, where alterations of the Golgi ribbon were also observed. Silencing and overexpression experiments revealed that SPCA1 affects cell morphology, Golgi apparatus integrity, and phagocytic functions. Since SPCA1 is also an efficient Mn2+ transporter and considering that Mn2+ excess causes manganism in the brain, we addressed the role of microglial SPCA1 in Mn2+ toxicity. Our results revealed a clear effect of Mn2+ excess on the viability and morphology of microglia. Subcellular analysis showed Golgi fragmentation and subsequent alteration of SPCA1 distribution from early stages of toxicity. Removal of Mn2+ by washing improved the culture viability, although it did not effectively reverse Golgi fragmentation. Interestingly, pretreatment with curcumin maintained microglia cultures viable, prevented Mn2+-induced Golgi fragmentation, and preserved SPCA Ca2+-dependent activity, suggesting curcumin as a potential protective agent against Mn2+-induced Golgi alterations in microglia.es_ES
dc.description.sponsorshipFEDER-Junta de Andalucía, Grant/Award Number: A1-CTS-324-UGR18es_ES
dc.description.sponsorshipUGR Research Program, Grant/Award Number: PP2022. PP.29es_ES
dc.language.isoenges_ES
dc.publisherWiley Online Libraryes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectbrain es_ES
dc.subjectcalciumes_ES
dc.subjectGolgies_ES
dc.titleUpregulation of the secretory pathway Ca2+/Mn2+-ATPase isoform 1 in LPS-stimulated microglia and its involvement in Mn2+-induced Golgi fragmentationes_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1002/glia.24528
dc.type.hasVersionVoRes_ES


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