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dc.contributor.authorFernández-Moya, Alejandro
dc.contributor.authorOviedo, Bielca
dc.contributor.authorLiempi, Ana
dc.contributor.authorGuerrero Muñóz, Jesús
dc.contributor.authorRivas, Cristian
dc.contributor.authorArregui, Roc
dc.contributor.authorAraneda, Sebastian
dc.contributor.authorCornet Gómez, Alberto 
dc.contributor.authorMaya, Juan Diego
dc.contributor.authorMüller, Marioly
dc.contributor.authorOsuna Carrillo De Albornoz, Antonio 
dc.contributor.authorCastillo, Christian
dc.contributor.authorKemmerling, Ulrike
dc.date.accessioned2024-11-12T10:59:58Z
dc.date.available2024-11-12T10:59:58Z
dc.date.issued2024-10-14
dc.identifier.citationFernández Moya, A. et. al. Front. Cell. Infect. Microbiol. 14:1437339. [https://doi.org/10.3389/fcimb.2024.1437339]es_ES
dc.identifier.urihttps://hdl.handle.net/10481/96868
dc.description.abstractTrypanosoma cruzi, the causative agent of Chagas disease, can be congenitally transmitted by crossing the placental barrier. This study investigates the role of T. cruzi-derived exovesicles (TcEVs) in facilitating parasite infection and the consequent tissue damage and apoptotic cell death in human placental explants (HPEs). Our findings demonstrate that TcEVs significantly enhance the parasite load and induce tissue damage in HPEs, both in the presence and absence of the parasite. Through histopathological and immunohistochemical analyses, we show that TcEVs alone can disrupt the placental barrier, affecting the basal membrane and villous stroma. The induction of apoptotic cell death is evidenced by DNA fragmentation, caspase 8 and 3, and p18 fragment immunodetection. This damage is exacerbated when TcEVs are combined with T. cruzi infection. These findings suggest that TcEVs play a critical role in the pathogenesis of congenital Chagas disease by disrupting the placental barrier and facilitating parasite transmission to the fetus. This study provides new insights into the mechanisms of transplacental transmission of T. cruzi and highlights the potential of targeting TcEVs as a therapeutic strategy against congenital Chagas disease.es_ES
dc.description.sponsorshipNational Fund for Scientific and Technological Development (FONDECYT; grant numbers 1220105, 1210159, and 11220310, respectively)es_ES
dc.description.sponsorshipPhD scholarship from the same agency (Beca ANID 21201823)es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectTrypanosoma cruzies_ES
dc.subjectplacenta es_ES
dc.subjecttissue damagees_ES
dc.subjectinfection es_ES
dc.subjectexovesicleses_ES
dc.titleTrypanosoma cruzi-derived exovesicles contribute to parasite infection, tissue damage, and apoptotic cell death during ex vivo infection of human placental explantses_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.3389/fcimb.2024.1437339
dc.type.hasVersionVoRes_ES


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