Design of innovative and low-cost dopamine-biotin conjugate sensor for the efficient detection of protein and cancer cells
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Notarbartolo, Monica; Alfieri, Maria Laura; Avolio, Roberto; Ball, Vincent; Errico, Maria Emanuela; Massaro, Marina; Puglisi, Roberta; Sánchez Espejo, Rita María; Viseras Iborra, César Antonio; Riela, SerenaEditorial
Elsevier
Materia
Dopamine Biotin Dip-coating Low-cost sensor
Date
2024-09-21Referencia bibliográfica
Notarbartolo, M. et. al. 6 78 (2025) 766–775. [https://doi.org/10.1016/j.jcis.2024.09.145]
Sponsorship
PJ_UTILE_2022VQR_Misura_B_D15_Notarbartolo of University of PalermoAbstract
The rapid, precise identification and quantification of specific biomarkers, toxins, or pathogens is currently a key strategy for achieving more efficient diagnoses. Herein a dopamine-biotin monomer was synthetized and oxidized in the presence of hexamethylenediamine, to obtain adhesive coatings based on polydopamine-biotin (PDA-BT) on different materials to be used in targeted molecular therapy. Insight into the structure of the PDA-BT coating was obtained by solid-state 13C NMR spectroscopy acquired, for the first time, directly onto the coating, deposited on alumina spheres. The receptor binding capacity of the PDA-BT coating toward 4-hydroxyazobenzene-2-carboxylic acid/Avidin complex was verified by means of UV–vis spectroscopy. Different deposition cycles of avidin onto the PDA-BT coating by layer-by-layer assembly showed that the film retains its receptor binding capacity for at least eight consecutive cycles. Finally, the feasibility of PDA-BT coating to recognize cell lines with different grade of overexpression of biotin receptors (BR) was investigated by tumor cell capture experiments by using MCF-7 (BR+) and HL-60 (BR−) cell lines. The results show that the developed system can selectively capture MCF-7 cells indicating that it could represent a first approach for the development of future more sophisticated biosensors easily accessible, low cost and recyclable with the dual and rapid detection of both proteins and cells.