Advanced Imaging Methodology in Bacterial Biofilms with a Fluorescent Enzymatic Sensor for pepN Activity
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Valverde Pozo, Javier; Paredes Martínez, José Manuel; García Rubiño, María Eugenia; Girón González, María Dolores; Salto González, Rafael; Álvarez Pez, José María; Talavera Rodríguez, Eva MaríaEditorial
MDPI
Materia
Bacterial biofilms PepN activity Bacterial processes
Date
2024-09-03Referencia bibliográfica
Valverde-Pozo, J.; Paredes, J.M.; García-Rubiño, M.E.; Girón, M.D.; Salto, R.; Alvarez-Pez, J.M.; Talavera, E.M. Advanced Imaging Methodology in Bacterial Biofilms with a Fluorescent Enzymatic Sensor for pepN Activity. Biosensors 2024, 14, 424. https://doi.org/10.3390/bios14090424
Sponsorship
Consejería de Universidad, Investigación e Innovación and por la Unión Europea con cargo al Programa FEDER Andalucía 2021–2027, grant number C-CTS-147-UGR23; Ministerio de Ciencia, Innovación y Universidades, grant number PID2023-149650NB-I00Abstract
This research explores the use of the pepN activity fluorescent sensor DCM-Ala in bacterial
biofilms, emphasizing its significance due to the critical role of biofilms in various biological processes.
Advanced imaging techniques were employed to visualize pepN activity, introducing a novel
approach to examining biofilm maturity. We found that the overexpression of pepN increases the
ability of E. coli to form biofilm. The findings demonstrate varying levels of pepN activity throughout
biofilm development, suggesting potential applications in biofilm research and management. The
results indicate that the fluorescent emission from this sensor could serve as a reliable indicator
of biofilm maturity, and the imaging techniques developed could enhance our understanding and
control of biofilm-related processes. This work highlights the importance of innovative methods in
biofilm study and opens new avenues for utilizing chemical emissions in biofilm management.