Intestinal inflammation marker calprotectin regulates epithelial intestinal zinc metabolism and proliferation in mouse jejunal organoids
Metadatos
Mostrar el registro completo del ítemAutor
González, R.; Ceacero Heras, Diego; Tena Garitaonaindia, Mireia; Álvarez Mercado, Ana Isabel; Gámez Belmonte, María de los Reyes; Chazin, Walter; Sánchez De Medina López-Huertas, Fermín; Martínez Augustín, María OlgaEditorial
Elsevier
Materia
Intestinal inflammation markers Calprotectin Intestinal epithelium
Fecha
2024-04-08Referencia bibliográfica
González, R., et al. Intestinal inflammation marker calprotectin regulates epithelial intestinal zinc metabolism and proliferation in mouse jejunal organoids. Biomedicine & Pharmacotherapy 174 (2024) 116555 [10.1016/j.biopha.2024.116555]
Patrocinador
Grant PID2020-112768RB-I00 funded by MICIU/AEI/10.13039/501100011033; Instituto de Salud Carlos III (ISCIII), PI21/00952, co-funded by the European Union; Grants AAGR-468-UGR20 and P20–00695 funded by the Consejería de Universidad, Investigación e Innovación of Junta de Andalucía and by “ERDF A way of making Europe”; FPU fellowship program of the Ministry of Education; Plan propio of the University of Granada; Instituto de Salud Carlos III, Spain; US National Institutes of Health [grants R01 AI101171 and R01 AI127793]Resumen
Calprotectin (CP), a heterodimer of S100A8 and S100A9, is expressed by neutrophils and a number of innate
immune cells and is used widely as a marker of inflammation, particularly intestinal inflammation. CP is a ligand
for toll-like receptor 4 (TLR4) and the receptor for advanced glycation end products (RAGE). In addition, CP can
act as a microbial modulatory agent via a mechanism termed nutritional immunity, depending on metal binding,
most notably Zn2+. The effects on the intestinal epithelium are largely unknown. In this study we aimed to
characterize the effect of calprotectin on mouse jejunal organoids as a model epithelium, focusing on Zn2+
metabolism and cell proliferation. CP addition upregulated the expression of the Zn2+ absorptive transporter
Slc39a4 and of methallothionein Mt1 in a Zn2+-sensitive manner, while downregulating the expression of the
Zn2+ exporter Slc30a2 and of methallothionein 2 (Mt2). These effects were greatly attenuated with a CP variant
lacking the metal binding capacity. Globally, these observations indicate adaptation to low Zn2+ levels. CP had
antiproliferative effects and reduced the expression of proliferative and stemness genes in jejunal organoids,
effects that were largely independent of Zn2+ chelation. In addition, CP induced apoptosis modestly and
modulated antimicrobial gene expression. CP had no effect on epithelial differentiation. Overall, CP exerts
modulatory effects in murine jejunal organoids that are in part related to Zn2+ sequestration and partially
reproduced in vivo, supporting the validity of mouse jejunal organoids as a model for mouse epithelium.