Evaluation of the usefulness of insertion‑null markers in critical skeletal remains
Metadatos
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Haarkötter Cardoso, Christian; Sáiz Guinaldo, María; Gálvez Escolano, Xiomara; Vinueza Espinosa, Diana C.; Medina Lozano, María Isabel; Álvarez Merino, Juan Carlos; Lorente Acosta, José AntonioEditorial
Springer Nature
Materia
Forensic Genetics Human remains INNULs
Fecha
2024-03-20Referencia bibliográfica
Haarkötter, C., Saiz, M., Gálvez, X. et al. Evaluation of the usefulness of insertion-null markers in critical skeletal remains. Int J Legal Med (2024). https://doi.org/10.1007/s00414-024-03205-3
Patrocinador
Funding for open access publishing: Universidad de Granada/CBUA.; Spanish Ministry of Universities for funding the development of his PhD (FPU 20/01967)Resumen
Forensic DNA analysis in compromised skeletal remains may pose challenges due to DNA degradation, often resulting
in partial or negative autosomal STRs profiles. To address this issue, alternative approaches such as mitochondrial DNA
or SNPs typing may be employed; however, they are labour-intensive and costly. Insertion-null alleles (INNULs), short
interspersed nuclear elements, have been suggested as a valuable tool for human identification in challenging samples due
to their small amplicon size. A commercial kit including 20 INNULs markers along with amelogenin (InnoTyper® 21) has
been developed. This study assesses its utility using degraded skeletal remains, comparing the results obtained (the number
of detected alleles, RFU values, PHR, and the number of reportable markers) to those obtained using GlobalFiler™.
Subsequently, the random match probability of the two profiles for each sample was determined using Familias version 3
to evaluate the power of discrimination of the results obtained from each kit. In every sample, InnoTyper® 21 yielded more
alleles, higher RFU values, and a greater number of reportable loci. However, in most cases, both profiles were similarly
informative. In conclusion, InnoTyper® 21 serves as a valuable complement to the analysis of challenging samples in cases
where a poor or negative profile was obtained.