Structural and functional characterization of genes PYL‑PP2C‑SnRK2s in the ABA signalling pathway of Cucurbita pepo
Metadatos
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Iglesias Moya, Jessica; Benítez, Álvaro; Segura, María; Alonso, Sonsoles; Garrido Garrido, Dolores; Martínez, Cecilia; Jamilena, ManuelEditorial
BioMed Central Ltd
Materia
Zucchini squash Response to ABA Cold stress response
Fecha
2024-03-11Referencia bibliográfica
Iglesias-Moya, J., Benítez, Á., Segura, M. et al. Structural and functional characterization of genes PYL-PP2C-SnRK2s in the ABA signalling pathway of Cucurbita pepo. BMC Genomics 25, 268 (2024). https://doi.org/10.1186/s12864-024-10158-9
Patrocinador
Grants PID2020-118080RB-C21, UAL18-BIOB017-B, and P20_00327, funded by the Spanish Ministry of Science and Innovation, the University of Almería and Junta de Andalucía; FPI Scholarship Program from the Spanish Ministry of Science and Innovation; D.I scholarship programmer from MCI (DIN2018–010127) with the company Green Breeding Biotech S. L.Resumen
Background The core regulation of the abscisic acid (ABA) signalling pathway comprises the multigenic families PYL,
PP2C, and SnRK2. In this work, we conducted a genome-wide study of the components of these families in Cucurbita
pepo.
Results The bioinformatic analysis of the C. pepo genome resulted in the identification of 19 CpPYL, 102 CpPP2C
and 10 CpSnRK2 genes. The investigation of gene structure and protein motifs allowed to define 4 PYL, 13 PP2C and 3
SnRK2 subfamilies. RNA-seq analysis was used to determine the expression of these gene families in different plant
organs, as well as to detect their differential gene expression during germination, and in response to ABA and cold
stress in leaves. The specific tissue expression of some gene members indicated the relevant role of some ABA signalling
genes in plant development. Moreover, their differential expression under ABA treatment or cold stress revealed
those ABA signalling genes that responded to ABA, and those that were up- or down-regulated in response to cold
stress. A reduced number of genes responded to both treatments. Specific PYL-PP2C-SnRK2 genes that had potential
roles in germination were also detected, including those regulated early during the imbibition phase, those regulated
later during the embryo extension and radicle emergence phase, and those induced or repressed during the whole
germination process.
Conclusions The outcomes of this research open new research lines for agriculture and for assessing gene function
in future studies.