Comparison of several ELISA tests for detecting the presence of IgG and IgM against herpes simplex viruses
Identificadores
URI: https://hdl.handle.net/10481/91828Metadatos
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Microbios .
Fecha
2000-02Referencia bibliográfica
Gutiérrez J, Fernández F, Vergara MJ, Suárez S, Soto MJ, Maroto MC. Comparison of several ELISA tests for detecting the presence of IgG and IgM against herpes simplex viruses. Microbios. 2000;103(405):127-32.
Resumen
Four enzyme-linked immunosorbent assays designated test 1 (ETI-HSVK-G 1/2); test 2 (ETI-HSVK-M 1/2); test 3 (ETI-HSVK-G 2), and test 4 (BioElisa HSV2 IgG) were studied to evaluate different stages of herpes simplex virus (HSV) infection. Samples (50 sera and 14 cerebrospinal fluid) were included in four groups. Group 1 consisted of samples from patients with primary HSV infections; group 2 comprised samples from patients with recurrent HSV infections; group 3 were samples nonreactive to HSV; and group 4 were samples from patients with infections by other herpes viruses (4a, chickenpox; 4b, herpes zoster; and 4c, infectious mononucleosis by Epstein-Barr virus). The percentages of agreement between tests 1 and 2 were 100 and 72.1%, respectively. The total diagnostic values of tests 1 and 2 were: 100 and 50% sensitivity, respectively; and 100 and 89% specificity, respectively. Few positive results for HSV-2 infection were found, and so, tests 3 and 4 were not evaluated. The results of tests 3 and 4 for a chickenpox patient, and a herpes zoster patient were not in agreement.