Effects of temperature on bone tissue. Histological study of the changes in the bone matrix
Metadatos
Mostrar el registro completo del ítemAutor
Fernández Castillo, Rafael; H. Ubelaker, Douglas; Lorente Acosta, Jose Antonio; Cañadas de la Fuente, Guillermo A,Editorial
Forensic Science International
Fecha
2013-03-10Referencia bibliográfica
Castillo RF, Ubelaker DH, Acosta JA, de la Fuente GA. Effects of temperature on bone tissue. Histological study of the changes in the bone matrix. Forensic Sci Int. 2013 Mar 10;226(1-3):33-7.
Resumen
The analysis of burned human remains has given rise to many publications in the literature and has
caused great interest among forensic specialists and physical anthropologists due to the difficulty in its
analysis and interpretation. The main goal of this study has been to measure the changes that occur in
bone matrix as a consequence of the increased temperature and establishing categories of histological
morphology in relation to fire temperature. To this end, a total of 150 bone cylinders from the ilium
obtained by bone biopsy. These samples have been obtained from forensic cadavers and burned at
controlled temperatures between 100 and 1100 8C in an oven. The samples were fixed in methyl
methacrylate and stained with hematoxylin–eosin, Goldner’s trichrome and toluidine blue stains. The
samples were studied using an optical microscope at 100!. Our study classifies the morphological
changes that occur in bone matrix in four stages as a result of the temperature.