A preliminary study on the presence of extended-spectrum beta-lactamases (ESBL) in clinical isolates of Escherichia coli in Granada (Spain)
Identificadores
URI: https://hdl.handle.net/10481/90198Metadatos
Mostrar el registro completo del ítemAutor
Solorzano Puerto, Antonio; Gutiérrez Fernández, José; Fernández, F.; Soto, M.J.; Piédrola Angulo, GonzaloEditorial
Annals of microbiology
Materia
Enterobacteriaceae Beta-lactamases Antimicrobial agent
Fecha
2004-02Referencia bibliográfica
SORLÓZANO, A., GUTIÉRREZ, J., FERNÁNDEZ, F., SOTO, M., & PIÉDROLA, G. (2004). A preliminary study on the presence of extended-spectrum beta-lactamases (ESBL) in clinical isolates of Escherichia coli in Granada (Spain). Annals of Microbiology, 54(2), 227-232.
Resumen
- A preliminary study was conducted to detect extended spectrum beta-lacta mases (ESBLs) and to describe ESBL types in clinical isolates of Escherichia coli from
Granada (Spain). Sixty-two isolates were examined using the VITEK 2 system, disk dif fusion method and Epsilon test. Fourteen randomly selected isolates were subjected to
genetic analysis in order to detect the ESBL type (CTX-M-10, CTX-M-9 and SHV). ESBL
isoelectric points were determined by isoelectric focusing using crude extracts from soni cated cells. Out of the 62 isolates with ESBLs detected by the VITEK 2 system, 61
(98.4%) were confirmed to contain ESBLs by disk diffusion and/or Epsilon test. The pre valent ESBL type in E. coli was CTX-M-9.