Reliability of low-avidity IgG and of IgA in the diagnosis of primary infection by rubella virus with adaptation of a commercial test
Identificadores
URI: https://hdl.handle.net/10481/90086Metadatos
Mostrar el registro completo del ítemAutor
Gutiérrez Fernández, José; Rodríguez, M. J.; Ory, F. De; Piédrola Angulo, Gonzalo; Maroto Vela, María del CarmenEditorial
Wiley
Materia
Rubella virus Avidity IgG
Fecha
1999Referencia bibliográfica
Gutiérrez J, Rodríguez MJ, De Ory F, Piédrola G, Maroto MC. Reliability of low-avidity IgG and of IgA in the diagnosis of primary infection by rubella virus with adaptation of a commercial test. J Clin Lab Anal. 1999;13(1):1-4.
Resumen
The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modified commercial test using 8 molar urea (indirect ELISA, DiaSorin, Italy) and IgA was determined with a homemade indirect ELISA test. Twenty-five patients with recent primary infection by rubella virus (group I) and 50 healthy subjects (group II) were studied. In group I low-avidity IgG varied between 100 and 0% (67.3+/-21.8%); IgA was present in 24 patients (96%). In group II low-avidity IgG varied from 50.4 to 0% (19.8+/-16.9%). IgA was present in 2 subjects (4%). The sensitivity of the Avi-EIA and the IgA test was 92 and 96%, respectively; specificity was 100 and 96%, respectively. We conclude that both low-avidity IgG and IgA tests are helpful and reliable for the diagnosis of recent primary infection.