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dc.contributor.authorCampos Sánchez, Fernando 
dc.contributor.authorBonhome Espinosa, Ana Belén 
dc.contributor.authorVizcaíno, G
dc.contributor.authorRodríguez, IA
dc.contributor.authorDurán-Herrera, D
dc.contributor.authorLópez López, Modesto Torcuato 
dc.contributor.authorSánchez-Montesinos García, Indalecio 
dc.contributor.authorAlaminos Mingorance, Miguel 
dc.contributor.authorSánchez Quevedo, María del Carmen 
dc.contributor.authorCarriel Araya, Víctor 
dc.date.accessioned2024-03-06T07:58:22Z
dc.date.available2024-03-06T07:58:22Z
dc.date.issued2018-02
dc.identifier.citationCampos F, Bonhome-Espinosa AB, Vizcaino G, Rodriguez IA, Duran-Herrera D, López-López MT, Sánchez-Montesinos I, Alaminos M, Sánchez-Quevedo MC, Carriel V. Generation of genipin cross-linked fibrin-agarose hydrogel tissue-like models for tissue engineering applications. Biomed Mater. 2018 Feb 8;13(2):025021. doi: 10.1088/1748-605X/aa9ad2es_ES
dc.identifier.urihttps://hdl.handle.net/10481/89813
dc.description.abstractGeneration of biomimetic and biocompatible artificial tissues is the basic research objective for tissue engineering (TE). In this sense, the biofabrication of scaffolds that resemble the tissues’ extracellular matrix (ECM) is an essential aim in this field. Uncompressed and nanostructured fibrin-agarose hydrogels (FAH and NFAH respectively) emerged as promising scaffold in TE, but its structure and biomechanical properties must be improved in order to broad their TE applications. Here we generated and characterized novel membranelike models with increased structural and biomechanical properties based on the chemical cross-linking of FAH and NFAH with genipin (GP at 0.1, 0.25, 0.5 and 0.75%). Furthermore, scaffolds were subjected to rheological (G, G’, G” modulus), ultrastructural and ex vivo biocompatibility analyses. Results showed that all GP concentrations increased the stiffness (G) and especially the elasticity (G’) of FAH and NFAH. Ultrastructural analyses demonstrated that GP and nanostructuration of FAH allowed controlling the porosity of FAH. In addition, biological studies revealed that higher concentration of GP significantly decreased the cell viability. Finally, this study demonstrated the possibility to generate natural FAH and NFAH with improved structural and biomechanical properties by using GP. However, further in vivo studies are needed in order to demonstrate the biocompatibility, biodegradability and regeneration capability of these cross-linked scaffolds.es_ES
dc.language.isoenges_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTissue Engineeringes_ES
dc.subjectNanostructuration techniquees_ES
dc.subjectGenipin Cross-linkinges_ES
dc.subjectFibrin-agarosees_ES
dc.subjectHydrogelses_ES
dc.subjectCell-biomaterials interactionses_ES
dc.subjectRheologyes_ES
dc.titleGeneration of genipin cross-linked fibrin-agarose hydrogel tissue-like models for tissue engineering applicationses_ES
dc.typejournal articlees_ES
dc.rights.accessRightsopen accesses_ES
dc.identifier.doi10.1088/1748-605X/aa9ad2
dc.type.hasVersionAMes_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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