Hyaluronate Nanoparticles as a Delivery System to Carry Neuroglobin to the Brain after Stroke
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Blanco, Santos; Peralta, Sebastián; Morales Hernández, María Encarnación; Castán, Herminia; Ruiz, María AdolfinaEditorial
MDPI
Fecha
2020-01Resumen
Therapies against stroke can restore the blood supply but cannot prevent the ischemic
damage nor stimulate the recovery of the infarcted zone. The neuroglobin protein plays an important
role in the neuro-regeneration process after stroke; however, the method for its e ective systemic
application hasnotbeenidentifiedyet, asneuroglobinisunabletopassthroughtheblood-brainbarrier.
Previously, we developed di erent types of sodium hyaluronate nanoparticles, which successfully
cross the blood-brain barrier after stroke. In this work, these nanoparticles have been used to
carry neuroglobin through the bloodstream to the nerve cells in rats submitted to stroke. We have
biosynthesized rat-recombinant neuroglobin and determined the formulation of sodium hyaluronate
nanoparticles loaded with neuroglobin, as well as its size and-potential, encapsulation e ciently,
in vitro release, and its kinetic of liberation. The results show that the formulation achieved is highly
compatible with pharmaceutical use and may act as a delivery system to transport neuroglobin within
the blood. We have found that this formulation injected intravenously immediately after stroke
reached the damaged cerebral parenchyma at early stages (2 h). Neuroglobin colocalizes with its
nanocarriers inside the nerve cells and remains after 24 h of reperfusion. In conclusion, the systemic
administration of neuroglobin linked to nanoparticles is a potential neuroprotective drug-delivery
strategy after stroke episodes.