PCR-free and chemistry-based technology for miR-21 rapid detection directly from tumour cells

Abstract

miRNAs play pivotal roles in various biological contexts, particularly in disease. Notably, miRNA-21 is implicated in severe pathological conditions, necessitating accurate quantification for medical diagnostics. Existing miRNA quantification techniques often fall short. Here, we introduce a novel, dynamic chemistry-based, PCR-free method for rapid and cost-effective miRNA-21 identification and quantification directly from tumor cells, employing FACS and fluorescent microplate assays. This approach, utilizing bead-based reagents, enables precise quantification of miR-21 molecules in MDA-MB-468 and H1975 tumor cells.