The L1Tc non-LTR retrotransposon of Trypanosoma cruzi contains an internal RNA-pol II-dependent promoter that strongly activates gene transcription and generates unspliced transcripts
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Rodríguez Heras, SaraEditorial
Oxford Univ Press
Fecha
2007Referencia bibliográfica
Heras SR, López MC, Olivares M, Thomas MC. The L1Tc non-LTR retrotransposon of Trypanosoma cruzi contains an internal RNA-pol II-dependent promoter that strongly activates gene transcription and generates unspliced transcripts. Nucleic Acids Res. 2007;35(7):2199-214. doi: 10.1093/nar/gkl1137. Epub 2007 Mar 16. PMID: 17369274; PMCID: PMC1874656.
Patrocinador
This work was supported by BMC2003–00834 from Plan Nacional IþDþI (MEC, Spain) and PAI ref. P05-CVI-01227 (Junta de Andalucı´a, Spain). S.R.H. was supported by a MEC Predoctoral Fellowship, Spain. Funding to pay the Open Access publication charge was provided by PAI CVI-155 (Junta de Andalucia, Spain).Resumen
L1Tc is the best represented autonomous LINE of
the Trypanosoma cruzi genome, throughout which
several functional copies may exist. In this study,
we show that the first 77 bp of L1Tc (Pr77)
(also present in the T. cruzi non-autonomous retrotransposon
NARTc, in the Trypanosoma brucei
RIME/ingi elements, and in the T. cruzi, T. brucei
and Leishmania major degenerate L1Tc/ingi-related
elements [DIREs]) behave as a promoter element
that activates gene transcription. The transcription
rate promoted by Pr77 is 10–14-fold higher than that
mediated by sequences located upstream from
the T. cruzi tandemly repeated genes KMP11 and
the GAPDH. The Pr77 promoter-derived mRNAs
initiate at nucleotide þ1 of L1Tc, are unspliced and
translated. L1Tc transcripts show a moderate half
life and are RNA pol II dependent. The presence
of an internal promoter at the 50 end of L1Tc favors
the production of full-length L1Tc RNAs and
reinforces the hypothesis that this mobile element
may be naturally autonomous in its transposition.