Effect of semi-permeable cover system on the bacterial diversity during sewage sludge composting
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Robledo Mahón, Tatiana; Aranda Ballesteros, Elisabet; Pesciaroli, Chiara; Rodriguez-Calvo, Alfonso; Silva-Castro, Gloria Andrea; González López, Jesús Juan; Calvo Sáinz, ConcepciónEditorial
Elsevier
Fecha
2018Patrocinador
This research was conducted with funding from Junta de Andalucía [Research project RNM-7370]. E.A would like to thank the Ministry of Economy and Competitiveness (MINECO) and FEDER funds for co-funding the Ram on y Cajal contract [RYC-2013- 12481]. We would like to acknowledge the Environmental Microbiology Research Group (RNM-270) of the University of Granada (Spain). The authors are grateful to Cinta G omez-Silv an from the Ecology Department of Lawrence National Berkeley Laboratory (California, US) for help with the statistical analyses and contributing valuable comments to the manuscript.We also wish to thank David Nesbitt for proof reading the document.Resumen
Sewage sludge composting is a profitable process economically viable and environmentally friendly. In
despite of there are several kind of composting types, the use of combined system of semipermeable
cover film and aeration air-floor is widely developed at industrial scale. However, the knowledge of the
linkages between microbial communities structure, enzyme activities and physico-chemical factors
under these conditions it has been poorly explored. Thus, the aim of this study was to investigate the
bacterial dynamic and community structure using next generation sequencing coupled to analyses of
microbial enzymatic activity and culturable dependent techniques in a full-scale real composting plant.
Sewage sludge composting process was conducted using a semi-permeable Gore-tex cover, in combination
with an air-insufflation system. The highest values of enzymatic activities such as dehydrogenase,
protease and arylsulphatase were detected in the first 5 days of composting; suggesting that during this
period of time a greater degrading activity of organic matter took place. Culturable bacteria identified
were in agreement with the bacteria found by massive sequencing technologies. The greatest bacterial
diversity was detected between days 15 and 30, with Actinomycetales and Bacillales being the predominant
orders at the beginning and end of the process. Bacillus was the most representative genus during
all the process. A strong correlation between abiotic factors as total organic content and organic matter
and enzymatic activities such as dehydrogenase, alkaline phosphatase, and ß-glucosidase activity was
found. Bacterial diversity was strongly influenced by the stage of the process, community-structure
change was concomitant with a temperature rise, rendering favorable conditions to stimulate microbial
activity and facilitate the change in the microbial community linked to the degradation process.
Moreover, results obtained confirmed that the use of semipermeable cover in the composting of sewage
sludge allow a noticeable reduction in the process-time comparing to conventional open windrows.