Antiproliferative, Antioxidant, Chemopreventive and Antiangiogenic Potential of Chromatographic Fractions from Anemonia sulcata with and without Its Symbiont Symbiodinium in Colorectal Cancer Therapy
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Peña Contreras, María Mercedes; Mesas Hernández, Cristina; Perazzoli, Gloria; Martínez Martínez, Rosario; Porres Foulquie, Jesús María; Doello, Kevin; Prados Salazar, José Carlos; Melguizo Alonso, Consolación; Cabeza Montilla, LauraEditorial
MDPI
Materia
Anemonia sulcata Symbiodinium Fractionation Antioxidant activity Chemopreventive activity Antiangiogenic activity Antitumor activity
Date
2023-07-08Referencia bibliográfica
Peña, M.; Mesas, C.; Perazzoli, G.; Martínez, R.; Porres, J.M.; Doello, K.; Prados, J.; Melguizo, C.; Cabeza, L. Antiproliferative, Antioxidant, Chemopreventive and Antiangiogenic Potential of Chromatographic Fractions from Anemonia sulcata with and without Its Symbiont Symbiodinium in Colorectal Cancer Therapy. Int. J. Mol. Sci. 2023, 24, 11249. [https://doi.org/10.3390/ijms241411249]
Sponsorship
Junta de Andalucía (project PYC20 RE 035 UGR); Junta de Andalucía (project P20_00540 and A-CTS-666-UGR20); Instituto Salud Carlos III (project PMPTA22/00136; PI19/01478) (FEDER program); AGR145 and CTS-107 (Andalusian Government); FPU2019 (Ref. FPU19/06170) grant from the Ministerio de Universidades (Spain)Abstract
Anemonia sulcata may be a source of marine natural products (MNPs) due to the antioxidant
and antitumor activity of its crude homogenates shown in vitro in colon cancer cells. A bioguided
chromatographic fractionation assay of crude Anemonia sulcata homogenates with and without its
symbiont Symbiodinium was performed to characterize their bioactive composition and further determine
their biological potential for the management of colorectal cancer (CRC). The 20% fractions
retained the in vitro antioxidant activity previously reported for homogenates. As such, activation
of antioxidant and detoxifying enzymes was also evaluated. The 40% fractions showed the greatest
antiproliferative activity in T84 cells, synergistic effects with 5-fluoruracil and oxaliplatin, overexpression
of apoptosis-related proteins, cytotoxicity on tumorspheres, and antiangiogenic activity. The
predominantly polar lipids and toxins tentatively identified in the 20% and 40% fractions could be
related to their biological activity in colon cancer cells although further characterizations of the active
fractions are necessary to isolate and purify the bioactive compounds.