Melatonin Preserves Fluidity in Cell and Mitochondrial Membranes against Hepatic Ischemia–Reperfusion
Metadatos
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MDPI
Materia
Ischemia-reperfusion injury Melatonin Antioxidant Membrane fluidity Carbonyl content Mitochondria
Fecha
2023-07-08Referencia bibliográfica
Esteban-Zubero, E.; López-Pingarrón, L.; Ramírez, J.M.; Reyes-Gonzales, M.C.; Azúa-Romeo, F.J.; Soria-Aznar, M.; Agil, A.; García, J.J.Melatonin Preserves Fluidity in Cell andMitochondrialMembranes against Hepatic Ischemia–Reperfusion. Biomedicines 2023, 11, 1940. [https:// doi.org/10.3390/biomedicines11071940]
Patrocinador
Gobierno de Aragón (grant No. B56_23D)Resumen
We evaluated the in vivo effects of melatonin treatment on oxidative damage in the liver
in an experimental model of ischemia–reperfusion. A total of 37 male Sprague-Dawley rats were
randomly divided into four groups: control, ischemia, ischemia + reperfusion, and ischemia + reperfusion
+ melatonin. Hepatic ischemia was maintained for 20 min, and the clamp was removed to
initiate vascular reperfusion for 30 min. Melatonin (50 mg/kg body weight) was intraperitoneally
administered. Fluidity was measured by polarization changes in 1-(4-trimethylammoniumphenyl)-
6-phenyl-1,3,5-hexatriene-p-toluene sulfonate). After 20 min of ischemia, no significant changes
were observed in cell and mitochondrial membrane fluidity levels, lipid peroxidation, and protein
carbonylation. However, after 30 min of reperfusion, membrane fluidity decreased compared to
controls. Increases in lipid and protein oxidation were also seen in hepatic homogenates of animals
exposed to reperfusion. Melatonin injected 30 min before ischemia and reperfusion fully
prevented membrane rigidity and both lipid and protein oxidation. Livers from ischemia–reperfusion
showed histopathological alterations and positive labeling with antibodies to oxidized lipids and
proteins. Melatonin reduced the severity of these morphological changes and protected against
in vivo ischemia–reperfusion-induced toxicity in the liver. Therefore, melatonin might be a candidate
for co-treatment for patients with hepatic vascular occlusion followed by reperfusion